Abstract
Abstract: A method has been developed for the in vitro synthesis of nearly complete double stranded DNA copies of polyA containing eukaryotic mRNAs. Amplification of in vitro synthesized DNA using molecular cloning techniques has provided large amounts of homogeneously pure eukaryotic gene sequences which can be studied directly or employed as hybridization probes for the identification and isolation of DNA sequences contiguous to structural genes in chromosomal DNA. The fidelity of in vitro synthesis and the stability of synthetic DNA in bacteria is demonstrated by the fact that the entire nucleotide sequence of a cloned DNA copy of rabbit β-globin mRNA is in complete agreement with available β-globin protein and mRNA sequence data. We are using the method of cDNA cloning to study the structure and chromosomal organization of a highly complex set of developmentally-regulated genes in the Silk Moth Antharaea polyphemus .
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
