Abstract
1. 1. A sensitive agar-diffusion slide method has been developed for the assay of ribonuclease activity in microvolumes of samples. 2. 2. The method involves the diffusion of ribonuclease from small wells cut into an RNA-agar-coated microscopic glass slide. 3. 3. Diffusion of ribonuclease results in the production of distinct zones of clearing around the wells after acid precipitation of the unhydrolysed RNA. 4. 4. Conditions for optimal ribonuclease activity on the agar-diffusion slides were determined and the assay was applied to crude tissue extracts.
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