Abstract
BackgroundPolycystic ovary syndrome (PCOS) is the most prevalent endocrinopathy in women of reproductive age. The study was commenced to assess the favorable effects of Rutin against metabolic, biochemical, histological, and androgenic aspects of polycystic ovary syndrome in rats.MethodsFemale Sprague-Dawley rats were administered letrozole (1 mg/kg) per orally (p.o) for a period of 21 days for the induction of PCOS, followed by dose of rutin (100 mg/kg and 150 mg/kg, p.o) for 15 days using 0.5% w/v CMC as vehicle. Metformin was also given as a standard control to one of the rat groups.Serum estradiol, progesterone, testosterone, serum lipid parameters, CRP and glucose levels were evaluated. Furthermore, antioxidant activity was tested using superoxide dismutase, catalase, glutathione per-oxidase and reactive-oxygen species level.ResultsRutin flavonoid had a dose-dependent effect on androgenic levels depicting more recovery in the rutin-I treated group, while rutin-II treated groups showed better antioxidant and lipid profiles as compared with PCOS groups. A decrease in the value of C reactive protein (CRP) and a restoration in the proportion of estrous phase smears were observed in the rutin treated groups. Histopathological examination of ovary revealed a significant decrease in the number of cystic follicles in post treated groups. The effects observed with rutin were moderately similar to that with standard metformin, a widely used treatment drug for PCOS.ConclusionThe study provides evidence for the potential ameliorative effects of rutin against clinical and biochemical features of PCOS.
Highlights
Polycystic ovary syndrome (PCOS) is the most prevalent endocrinopathy in women of reproductive age
Effect of metformin and rutin on anthropometrical parameters Administartion of letrozole to female rats for 36 days resulted in a significant increase in final body weights of PCOS group as compared to control (P < 0.05), all the other anthropometrical parameters including weight gain, AC, Total cholesterol (TC), AC/TC ratio, body mass index (BMI), Lee index and specific rate of body mass gain remained non-significant among rats in the various groups
Histopathological analysis The ovaries were processed for paraffin embedding, sectioned at 7 μm, stained with hematoxylin and eosin, and Effect of metformin and rutin on colpocytological examination Administration of letrozole to rats for 36 days caused disturbance of normal estrous cycle starting from day 17th till the end, in the PCOS group, resulting into a profuse vaginal secretion
Summary
The study was commenced to assess the favorable effects of Rutin against metabolic, biochemical, histological, and androgenic aspects of polycystic ovary syndrome in rats. Polycystic ovary syndrome (PCOS) is the most prevalent endocrinopathy in women of reproductive age. The absence of aromatase enzyme causes disturbance in the steroidogenesis, causing increase in the production of androgens developing PCOS [14]. Metformin is commonly known as Glucophage and it belongs to the biguanide class of drugs. It has the chemical formula of C4H11N5 and molecular weight of 129.1636 g/ mol. Metformin is a best drug for PCOS treatment [16]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call