Abstract
1. 1. RNA synthesis induced by a single intravenous dose of aldosterone in rat kidney was characterized by differential thermal phenol extraction, a procedure which partially separates transfer, ribosomal and messenger RNA. 2. 2. Aldosterone stimulates the incorporation of [ 14C]orotic acid into RNA fractions obtained at all temperatures. 3. 3. Stimulated incorporation was observed at 30 and 60 min after administration of aldosterone. 2–3 h after aldosterone administration, the incorporation of [ 14C]orotic acid into RNA was again approaching control levels. 4. 4. Gradient density analysis showed consistent aldosterone stimulation in the 28-, 18- and 4-S peaks of RNA. 5. 5. Phenylbutazone, a nonsteroidal antinatriuretic agent, did not appear to stimulate the incorporation of [ 14C]orotic acid into RNA.
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