AKRİLAMİDE MARUZ KALAN SIÇANLARIN KALP DOKUSU ÜZERİNDE SELENYUMUN KORUYUCU ROLÜ

Abstract

Objective
 Acrylamide (ACR) is a harmful organic reactive compound
 widely used in different industrial fields. Sele-
 nium (SEL) is a trace element that takes part in some
 chemical and enzymatic reactions in both animals
 and human organisms and can cause some disorders
 in many systems such as the cardiovascular
 system in case of deficiency. The aim of this study is
 to investigate whether SEL has a protective effect on
 cardiac tissue in experimentally induced ACR toxicity
 in rats.
 Material and Method
 A total of 28 rats were randomly and equally divided
 into four groups: Control, SEL, ACR, SEL + ACR. At
 the end of the study, cardiac markers such as creatine
 kinase MB (CKMB), lactate dehydrogenase (LDH)
 and ischemia-modified albumin (IMA) from blood
 samples were measured. Oxidative stress markers
 such as total oxidant status (TOS), total antioxidant
 status (TAS) and oxidative stress index (OSI) in heart
 tissue were analyzed. In addition, inducible nitric oxide
 synthase iNOS activities were determined in heart
 sections using immunohistochemical methods.
 Results
 The ACR + SEL group showed a significant decrease
 in TOS, OSI, CKMB, LDH, IMA levels and significant
 increase in TAS level compared to the ACR group.
 In histopathological and immunohistochemical evaluation
 of the heart sections; Disorganization in cardiac
 muscle fibers, increased connective tissue between
 muscle fibers and irregular eosinophilia in muscle
 fibers were detected in ACR group. A decrease in
 histopathological findings was observed in the SEL
 treated groups compared to the ACR group. iNOS
 immunoreactivities; moderate marking was observed
 in the ACR group sections, while poor marking was
 observed in the ACR + SEL group.
 Conclusion
 We determined that ACR caused damage to the heart
 tissue of rats through various metabolic pathways and
 that these damages were reversed in the groups given
 the SEL.