Abstract
A study was conducted on the effect of aflatoxin B 1 on protein phosphorylation in rat livers by incubation of soluble and insoluble cell fractions with [γ 32P] ATP. SDS-polyacrylamide gel electrophoresis indicated that a total of eight rat liver phosphoproteins were affected during a feeding period of 36 weeks on a carcinogenic aflatoxin B 1-containing diet compared to the phosphoprotein patterns obtained from the livers of rats on a normal noncarcinogenic diet. The appearance of only two of these phosphoproteins were cAMP dependent. DEAE-cellulose chromatography employed to separate the different histone kinase activities in soluble rat liver cell fractions, showed that the specific activity of histone kinase I activity was increased but its total activity decreased while the histone kinase II activity stayed unchanged for rats submitted to the aflatoxin B 1-containing diet compared to those on the normal noncarcinogenic diet.
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