Abstract
Porous membranes with glycopolymer brushes were prepared as biomaterials for affinity separation. Glycopolymer brushes contained acrylic acid and D-mannose or N-acetyl-D-glucosamine, and were formed on substrates by surface-initiated atom transfer radical polymerization. The presence of glycopolymer brush was confirmed by X-ray photoelectron spectroscopy, contact angle, and ellipsometry measurements. The interaction between lectin and the glycopolymer immobilized on glass slides was confirmed using fluorescent-labeled proteins. Glycopolymer-immobilized surfaces exhibited specific adsorption of the corresponding lectin, compared with bovine serum albumin. Lectins were continuously rejected by the glycopolymer-immobilized membranes. When the protein solution was permeated through the glycopolymer-immobilized membrane, bovine serum albumin was not adsorbed on the membrane surface. In contrast, concanavalin A and wheat germ agglutinin were rejected by membranes incorporating D-mannose or N-acetyl-D-glucosamine, respectively. The amounts of adsorbed concanavalin A and wheat germ agglutinin was increased five- and two-fold that of adsorbed bovine serum albumin, respectively.
Highlights
Saccharides on the surface of cell membranes play a role in cell-cell, cell-extracellular matrix, and cell-virus interactions [1,2]
Tsujii et al reported that soluble polymers concurrently synthesized in solution media could be used to estimate the physical properties of polymers grafted on surfaces [27]
ethyl 2-bromo isobutyrate (EBiB) as a soluble initiator was added to the reaction solution, and the resulting poly(glyco-MA) in solution was used for size exclusion chromatography (SEC) as an alternative to that immobilized on the surface (Figure S1 in Supplementary Information)
Summary
Saccharides on the surface of cell membranes play a role in cell-cell, cell-extracellular matrix, and cell-virus interactions [1,2]. Allows high-densely polymer brushes to be prepared on various materials, with controlled molecular weight [17,18,19,20,21]. Reversible-deactivation radical polymerizations involving ATRP have been applied for synthesis of glycopolymer and preparation of biomaterial [22]. Micro-filter and ultra-filter membranes are used to separate micrometer-sized bacteria and nanometer-sized proteins and viruses, respectively. When the glycopolymer is grafted on the membrane surface containing micro-scale pores, the target biomolecule is adsorbed because of convectional flow toward the saccharide ligand on the surface. Glycopolymer brushes were grafted on glass slides and micro-porous membranes by SI-ATRP. The specific interactions between glycopolymers containing Man and GlcNAc, and the lectins concanavalin A (Con A) and wheat germ agglutinin (WGA), respectively, were evaluated using fluorescence microarrays. Glycopolymer-immobilized membranes were used for the continuous separation of lectin
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