Abstract
Understanding of cell wall architecture, including the crosslinking of cell wall polymers, provides crucial information for elucidating the relationship between cell wall structure and cell function. Moreover, examination of the cell wall informs efforts to improve biomass breakdown in bioreactor conditions. Over the past decades, imaging techniques have been used extensively to reveal the structural organization and chemical composition of cell walls, but detailed imaging of the native composition and architecture of the cell wall remains challenging. Here, we review progress in the development of cell wall imaging techniques. In particular, we focus on several advanced, label-free techniques for imaging cell walls and their potential applications in investigation of the biological functions of plant cell walls.
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