Abstract
The chemical structure and composition of conidial cell walls of Cochliobolus miyabeanus were studied. Analyses by means of gas liquid chromatography, high performance liquid chromatography, enzymatic digestion and nuclear magnetic resonance and infrared absorption spectroscopy revealed that the acid-soluble neutral polysaccharide was composed of β-1, 6-and β-1, 3-linked glucosyl residues in the ratio of 2 : 3, that the branched units connected through C6 or C3 of the main-chain glucan, and that nonreducing termini existed in the ratio of one residue to five glycosyl residues. Amino acid analysis indicated the presence of glycine, alanine, aspartic acid, serine, leucine, glutamic acid, proline and threonine in decreasing order of contents. The acid-alkaliinsoluble polysaccharide was identified as chitin by enzymatic digestion, X-ray diffraction and infrared spectral analyses.
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