Advanced treatment of sewage by membrane bioreactor associate with genetically engineered autotrophic nitrifying bacteria

Abstract

This study introduced a process of MBR combing with genetically engineered bacteria of expressing nirs and ppk genes (GEB-Nirs/PPK) for advanced treatment of sewage in scenic area. An industrial scale application was staged anaerobic digestion, aerobic digestion. Over more than 150 days of continuous operation, TMP in this system was less than 0.18 bar. With a membrane flux of 6.48 m3/h, TMP remained below 0.2 bar and effluent remained above 70 m3 during continuous operation. Average COD and BOD removals averaged 94.2% and 93.6%, and were obtained with average effluent COD and BOD below 10.4 mg/L and 4.2 mg/L, respectively. The TN and TP removals averaged 98.8% and 94.3%, and never higher than 3.2 mg/L and 0.2 mg/L, respectively, in the processing system. In conclusion, these results indicate that the process of MBR associate with genetically engineered autotrophic nitrifying bacteria is of high-efficiency for advanced treatment of sewage.