Abstract
High blood glucose levels can cause fatal complications, one of which is gangrene. Gangrene is a chronic condition and very difficult to cure. The final solution that will be carried out is amputation so as not to cause a more serious condition. Treatment for diabetic gangrene in general is by administering broad spectrum antibiotics. The use of antibiotics is starting to experience resistance, so alternatives to traditional drug therapy are being developed to suppress the occurrence of resistance. The results of the phytochemical screening of the ethyl acetate fraction of the Kadara seeds contained both hydrophilic and lipophilic saponin and flavonoid compounds. Flavonoids function as anti-fungal, anti-septic and cell regeneration or repair, while saponins can stimulate collagen growth and stimulate the growth of new cells. This research is a development of previous research on the effectiveness of the SNEDDS formula for Ethylacetate Fraction of Kadara Fruit Seed Flesh Extract against S.epidermidis and E.coli bacteria that cause diabetic gangrene in vitro. To facilitate the delivery of SNEDDS preparations, a topical preparation formulation is needed, one of which is Hydrogel. This study aims to determine the effect of administering SNEDDS hydrogel ethyl acetate fraction of Kadara fruit seed extract on blood sugar levels and the extent of wound healing in diabetic rats based on the distribution of collagen tissue through histopathological observations. This research began with maceration to obtain a thick extract, followed by fractionation and making SNEDDS hydrogel preparations. Evaluation of preparations and activity tests is carried out. Research results show that the SNEDDS hydrogel fraction of ethyl acetate fruit seed extract can reduce blood sugar on day 10 but is still in the hyperglycemic category from 487 mg/dL to 286 mg/dL. The density of collagen connective tissue formed by SNEDDS hydrogel from the ethyl acetate fraction was the highest (57.07%), with positive control (41.28%) and negative control (17.93%). Statistical data shows that the positive control group is significantly different from the negative control with a value of p= 0.025<0.05 and the positive control group is not significantly different from the SNEDDS hydrogel fraction ethyl acetate group with a value of p=0.166>0.05. Meanwhile, the SNEDDS hydrogel group with the ethyl acetate fraction was significantly different from the negative group, p value = 0.003 <0.005.
Published Version
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