Abstract
Diabetes mellitus (DM) is a metabolic disease which is becoming the first number of health problem in Indonesia, based on the results of the Basic Health Research of Ministry of Health in 2013. One of the diabetes complications affected by high levels of blood glucose is diabetic ulcers wich 85% the number of cases was overed by amputation as the result of improper handling. Herbal treatments could be an alternative treatment of diabetic ulcers, one of them is binahong plant. The results of previous studies have shown the ability of ethanolic extract of binahong leaf in accelerating wound healing in diabetic rats. This study would be a continued study to test five kinds of binahong leaf fractions (FDB) with gradual solvent polarity in accelerating wound healing in diabetic ulcer. Wound healing parameters observed were percentage of wound healing from the wound diameter contraction. The test begins with ethanolic extract of binahong leaf fractionation to produce fractions of hexane (FHDB), chloroform (FKDB), ethyl acetate (FEADB), and ethanol (FEDB). Water fraction (FADB) was obtained from ethanolic leaf extract residues of binahong leaf extraction. Each fraction activity was tested topically twice daily on dorsal of test animals that created wounds using a punch biopsy 5 mm diameters. Grouping of test animals were divided into 13 groups with normal control group (non diabetes), negative control (diabetes + topical application of biocream®), positive control (diabetes + topical application of madecassol), and 10 diabetes groups with topical application of 5 kinds of binahong leaf fractions with each fractions consist of a concentrations of 5% and 10% with biocream® as a vehicle. Diabetic parameters measured include blood glucose levels (KGD) and weight lost percentage (PB%) in day 1 and 10 during a 10-day treatment. The results showed the influence of KGD in the condition of diabetes on wound healing rats diabetic ulcers which topically treated with binahong leaf fractions. FADB 10% has significant differences potention in wound healing process in diabetic rats macroscopically that accelerates wound contraction compared with negative and positive control groups (p<0,05).
Highlights
Diabetes mellitus (DM) is a metabolic disease which is becoming the first number of health problem in Indonesia, based on the results of the Basic Health Research of Ministry of Health in 2013
The results showed the influence of kadar glukosa darah (KGD) in the condition of diabetes on wound healing rats diabetic ulcers which topically treated with binahong leaf fractions
Mechanisms and pharmacology of diabetic neuropathy– experimental and clinical studies
Summary
Pemeriksaan kadar glukosa darah (KGD) puasa dilakukan sebelum induksi STZ, 3 hari setelah induksi STZ (Nayak et al, 2007), dan 2x selama perlakuan pada hari ke 1 dan 10 pemberian aplikasi topikal 5 jenis fraksi daun binahong. Pembuatan Luka pada Hewan Uji Setelah 3 hari pasca induksi STZ dosis tunggal 45 mg/kgBB, hewan uji dengan KGD di atas 200 mg/dL digunakan untuk perlakuan hewan uji diabetes. Selanjutnya dilakukan pembuatan luka yang diawali dengan anestesi hewan uji dengan ketamin dosis 50 mg/kgBB i.m. Setelah hewan uji kehilangan kesadaran, area. Pengelompokan Hewan Uji Perlakuan pada hewan uji tikus dibagi menjadi 13 kelompok, dengan 1 kelompok non diabetes dan 12 kelompok diabetes yaitu kontrol normal: non diabetes, tanpa pemberian obat, kontrol negatif : diabetes + aplikasi topikal biocream®, kontrol positif : diabetes + aplikasi topikal madecasol, dan kelompok 10 Fraksi : diabetes + aplikasi topikal fraksi. Pemberian aplikasi topikal dilakukan selama 10 hari setelah pembuatan luka. Perbedaan bermakna jika signifikansi kurang dari taraf kesalahan α (p
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