Action of enamel matrix proteins on human osteogenous progenitor bone marrow cells, an experimental study

G Biloklytska,L Panchenko,I Braun

doi:10.22141/2306-2436.5.1-2.2016.121284

Abstract

Complex treatment of moderate and severe generalized periodontitis includes surgical phase of treatment in aim to provide regeneration and long-term disease remission. The obtained results confirm presence of osteoinductive properties in enamel matrix proteins, this data may be useful during surgical procedures on periodontal tissues.

Highlights

Periodontal diseases (PD) are prevalent diseases among other dental pathologies and are still substantial public burden worldwide [2,3,13,14,36]
A major discovery was the finding that periodontal tissue regeneration could be achieved by application of enamel matrix proteins (EMPs), and later – by the FDA approved medical device termed Emdogain [9,10]
Ameloginin expression is highest in bone cells adjacent to periodontal ligament (PDL) and in the periosteal cells compared to bone cells distal to the PDL and the periosteum [11,12]

Summary

Introduction

Periodontal diseases (PD) are prevalent diseases among other dental pathologies and are still substantial public burden worldwide [2,3,13,14,36]. Current data indicate that amelogenin stimulates a cascade of events enhancing remodelling and regeneration of periodontal tissues [13,14] Another investigations showed the expression of amelogenin mRNA isoform-M180 in macrophages, suggesting the endogenous expression of amelognin in osteoclasts [3]. Amelogenin was highly expressed in endosteal cells, osteoblasts, and cells surrounding blood vessels in the regenerated area and in much less extent in bone cells distal to the regenerated area [12] It was not clear which types of the EMPs induce the regeneration, and what molecular mechanisms are involved [34]. Treatment with EMD of human osteoblastic cells (SaM-1) from one patient [28] and of rat femoral bone marrow stroma [20], mouse preosteoblastic cell line [15], significantly stimulated cell proliferation. The increased expression of BMP-2, BMP-7, BSP, cementum attachment protein-23

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Conclusion