Abstract
Action of heated RNAs from reticulocytes and liver nuclei on the cell-free synthesis of hemoglobin RNAs from rabbit reticulocytes and liver nuclei and from guinea-pig liver nuclei, as well as the fractions obtained from these RNAs by centrifugation in a sucrose gradient, have been heated at 90° for 30 min and added to cell-free systems from reticulocytes in order to observe their action on hemoglobin biosynthesis. 1. 1. Heating increases the stimulating effect of reticulocyte RNA on hemoglobin biosynthesis; it very slightly increases the stimulating effect of rabbit liver RNA and has no effect on the RNA from guinea-pig liver. 2. 2. Heating of ribosomal RNAs from reticulocytes suppresses their inhibiting effect on hemoglobin biosynthesis; it has little effect on ribosomal RNA from rabbit liver and no effect on ribosomal RNA from guinea-pig liver. 3. 3. Heating of RNAs from rabbit reticulocytes and liver which sediment between 16 S and 4 S, and which probably contain messenger RNA for hemoglobin, does not modify their stimulating effects on hemoglobin biosynthesis. 4. 4. If messenger RNA is removed from reticulocyte ribosomes by elimination of magnesium, the 28-S RNA prepared from these ribosomes is not modified by heating. If this 28-S RNA is incubated with a preparation rich in messenger RNA and reisolated, it becomes an activator of hemoglobin synthesis by heating. These observations can be explained by the existence of specific bonds between the messenger RNA of rabbit hemoglobin and ribosomal RNA from rabbit reticulocytes. These is little affinity between this messenger RNA and ribosomal RNA from rabbit liver, and no affinity between this messenger RNA and ribosomal RNA from guinea pig.
Published Version
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