Abstract
An accurate, precise, and simple method for the analysis of thiamine has been developed. The method is based on the salt formation of thiamine with an acid dye, bromothymol blue. There is no interference from other vitamins, hormones (ethinyl estradiol and progesterone), and excipients. The nature of reaction between thiamine and bromothymol blue appears to be a simple acid-base reaction. The stoichiometric balance has been established to be one part of dye to one part of thiamine.
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