Abstract 5417: Improved tumor targeting by BLD1-ELP

Abstract

Abstract Elastin-like polypeptide (ELP) is a promising class of recombinant biopolymers for drug delivery to solid tumors. It is a thermally responsive polypeptide derived from mammalian elastin and consists of repeating pentapeptide sequence, Val-Pro-Gly-X-Gly, where X can be any amino acid except proline. ELPs conjugated to targeting peptide and drugs would enable enhanced targeting for drug delivery to solid tumors. BLD1 is a peptide which can selectively bind to bladder tumor cells. Not only for overcoming its rapid elimination from circulation and short half-life, but also enhancing tumor targeting efficiency, we introduced multiple BLD1 into the ELP (BLD1-ELP). Transition temperature (Tt) of BLD1-ELP was approximately 39 °C. BLD1-ELP showed superior cell binding ability than BLD1 peptide alone with 5637 and HT-1376 bladder tumor cell lines. The confocal microscopic imaging further confirmed that BLD1-ELP polymer efficiently bound to 5637 cells and HT-1376 cells compare to HEK293 embryonic kidney epithelial cells. The cellular uptake of BLD1-ELP was also much higher in 5637 cells and HT-1376 cells than in HT-29 colon cancer cells and HEK293 cells. In in vivo imaging analysis, tumor targeting of intravenously injected BLD1-ELP was observed in 5637 tumor xenograft model. These data suggest that BLD1-ELP can be developed as an effective tumor targeting, drug delivery system for diagnosis and therapy. Citation Format: Eun-A Cho, Vijaya Devi Sarangthem, Byung-Heon Lee, In-San Kim, Rang-Woon Park. Improved tumor targeting by BLD1-ELP. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5417. doi:10.1158/1538-7445.AM2014-5417