Abstract 4489: Targeting cancer cells and antitumor effects of brucine immunonanoparticles on hepatocellular carcinoma in vivo

Abstract

Abstract Background: In vitro and in vivo studies have showed that brucine can inhibit the growth of liver cancer cells and animals with bearing cancer , and may be a promising anti-cancer drugs. However, high toxicity, poor water solubility, short half-life, narrow therapeutic window, close therapeutic and toxic dose limit its clinical application in malignant tumor treatment. In this study, we report on the preparation , in vivo distribution and antitumor effect of the brucine immuno-nanoparticles. Methods: Anionic polymerization and chemical modification technology was used to prepare carboxylated polyethylene glycol-poly lactic acid copolymer carrier material, phacoemulsification technology was employed to prepare carboxylated polyethylene glycol-polylactic acid copolymer brucine nanoparticles and chemical coupling technology was utilized to develop anti-human AFP McAb-polyethylene glycol-poly lactic acid copolymer brucine immuno-nanoparticles. The nude mice in situ transplanted liver cancer model was established by surgical technique, and the brucine immuno-nanoparcitles was injected by caudal vein at indicated time. The liver and kidney function and AFP level were assayed , and the anti-tumor effect was observed after intravenous administration of the brucine immuno-nanoparticles. Results:This results showed that in vivo application of brucine immuno-nanoparticles caused temporary liver and kidney functions damage and significantly reduced AFP secretion. Brucine immuno-nanoparticles had good slow releasing and tumor targeting properties. The brucine immuno-nanoparticles inhibited CD34 expression and angiogenesis of tumor tissues, induced tumor apoptosis, and inhibited tumor growth. Conclusions: The brucine immuno-nanoparticles has good tumor targeting and obvious anti-tumor effects , and is a promising targeted drug for liver cancer therapy in the future. Note: This abstract was not presented at the meeting. Citation Format: Jianmin Qin. Targeting cancer cells and antitumor effects of brucine immunonanoparticles on hepatocellular carcinoma in vivo. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4489. doi:10.1158/1538-7445.AM2014-4489