Abstract 2816: The Jackson Laboratory Repository: Mouse models for cancer research

Abstract

Abstract The mouse is a well-established model organism for research of the biology, prevention, diagnosis, and treatment of cancer. Among the advantages of this valuable biomedical research tool are a relatively short reproductive cycle, an accelerated lifespan, and the ease of genetic manipulation and modification. Serving as a resource to the scientific community, the JAX Repository collection encompasses over 10,000 mouse strains and acquires hundreds of new models each year. Some of these new models are highlighted in this poster. Many of these strains have applications for modeling human cancer, including genetically engineered mouse models for specific cancers and xenograft models. Research tool strains, such as cas9-expressing (CRISPR) lines, a suite of immunodeficient mice, and recombinase expressing strains, are available to investigators to generate customized mutant lines for specialized purposes. JAX offers supplementary research tools that include specialized strain platforms and other useful resources. The JAX patient-derived xenograft (PDX) cancer model resource leverages the immunodeficient NSG strain for tumor engraftment. The Repository maintains a searchable online resource to find mice (www.jaxmice.jax.org) and an Oncology Therapeutic Area page (www.jax.org/jax-mice-and-services/solutions-by-therapeutic-area/oncology) as a launch pad to featured JAX strains and resources related to oncology research. The Repository has a rigorous quality control program that inspects and confirms expected mutation identity and genetic background. JAX mouse lines are also screened for alleles (GFP, cre, lacZ, etc.) that may contaminate strains in mouse lines shared between labs—an underappreciated problem that may contribute to inconsistencies of reproducibility. In addition, the Reproductive Sciences group at JAX safeguards each strain by cryopreservation, and can create cohorts for studies rapidly using IVF technology. Researchers can submit their strains to be considered for inclusion in the Repository by using the on-line form available at The Jackson Laboratory website: www.jax.org/donate-a-mouse. The Jackson Laboratory Repository is supported by the NIH, The Howard Hughes Medical Institute, and other private charitable foundations. Citation Format: Deborah Boswell, Stephen Rockwood, Michael Sasner, The JAX Repository Team. The Jackson Laboratory Repository: Mouse models for cancer research [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 2816. doi:10.1158/1538-7445.AM2017-2816