Abstract A05: The Mouse Tumor Biology (MTB) Database: An electronic tool for identifying and evaluating mouse and PDX models of human cancer

Abstract

Abstract The increasing number and diversity of available mouse models of human cancer and their growing importance in scientific research have resulted in an enormous increase in the amount and types of data generated from these models. These models represent powerful tools for studying biological and genetic mechanisms of cancer and for translation into potential clinical therapeutics. However, the amount of available data makes it challenging to identify and evaluate specific models and data important for an individual laboratory's research. Placing these data in their proper genetic context is crucial to understanding the biochemical and molecular mechanisms of initiation, progression, and metastasis of different cancers. In addition, the ability of the immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mouse strain to host patient derived xenograft (PDX) models only increases the number of available models and the data produced from them. The Mouse Tumor Biology (MTB) Database provides access to data from mouse and PDX models of human tumors and the tools to analyze these data, facilitating the discovery and evaluation of novel mouse and PDX models of human cancers (http://tumor.informatics.jax.org). MTB includes data on endogenously arising tumors (both spontaneous and induced) in genetically defined mice (inbred, hybrid, mutant, and genetically engineered mice) and information from PDX models of human tumors and provides freely available web access to these data. MTB integrates data from peer-reviewed literature, laboratories studying mouse models of human cancer, production mouse colonies at The Jackson Laboratory (JAX), colonies of aging mice from the Jackson Aging Center, and PDX data from the Jackson Laboratory Patient-derived xenograft resource. MTB also incorporates data from PathBase, and mouse gene expression data sets from NCBI's Gene Expression Omnibus (GEO) and the Array Express Database. Data include tumor classification, incidence and latency, tumor associated quantitative trait loci (QTL), pathology reports, images and genetic changes in tumors (somatic) and background strain (germline). Data type specific query forms (tumor, genetic etc.) allow detailed searches. MTB also can be searched using human gene symbols for orthologous mouse genes and associated data. Pathology images are submitted by the scientific community, from primary literature (with publisher permission), and from JAX colonies. MTB also includes immunohistochemistry data on over 500 antibodies with accompanying images of positive control samples and links to the respective vendors. MTB encourages direct submission of mouse tumor data and images from the cancer research community and has developed a web-based system to facilitate submission of data. Standard nomenclature, controlled vocabularies and literature citations facilitate data integration and robust searches. MTB is integrated with the Mouse Genome Informatics resource (MGI, http://www.informatics.jax.org) and provides links to other related online resources such as the Mouse Phenome Database (MPD), the Biology of the Mammary Gland Web Site, and the NCI Mouse Repository. MTB is supported by NCI grant CA089713. Citation Format: Dale A. Begley, Debra M. Krupke, Steven B. Neuhauser, Joel E. Richardson, John P. Sundberg, Carol J. Bult, Janan T. Eppig. The Mouse Tumor Biology (MTB) Database: An electronic tool for identifying and evaluating mouse and PDX models of human cancer. [abstract]. In: Proceedings of the AACR Special Conference: The Translational Impact of Model Organisms in Cancer; Nov 5-8, 2013; San Diego, CA. Philadelphia (PA): AACR; Mol Cancer Res 2014;12(11 Suppl):Abstract nr A05.