A validated HPTLC method for determination of ondansetron in combination with omeprazole or rabeprazole in solid dosage form

Manisha Puranik,Sj Wadher,Pg Yeole,Pb Raval

doi:10.4103/0250-474x.43011

Abstract

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed for the simultaneous estimation of ondansetron combinations in solid dosage form with omeprazole and rabeprazole, respectively. The method involved separation of components by TLC on a precoated silica gel 60 F254 using a mixture of dichloromethane:methanol (9:1) as a mobile phase. Detection of spots was carried out at 309 nm and 294 nm for ondansetron with omeprazole and ondansetron with rabeprazole combinations, respectively. The mean retardation factor for ondansetron and omeprazole were found to be 0.42±0.02, 0.54±0.03, respectively while for ondansetron and rabeprazole, 0.41± 0.02 and 0.51±0.02, respectively. The linearity and range was 0.1 to 0.5 μg/spot for three drugs. The method was validated for precision, accuracy and reproducibility.

Highlights

Camag HPTLC system comprising of Camag linomatV semiautomatic sample applicator, Camag TLC scanner 3, Camag Win CATS software, Camag twin trough chamber and a sonicator were used
A Validated HPTLC Method for Determination of Ondansetron in Combination with Omeprazole or Rabeprazole in Solid Dosage Form
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed for the simultaneous estimation of ondansetron combinations in solid dosage form with omeprazole and rabeprazole, respectively

Summary

Camag HPTLC system comprising of Camag linomat

V semiautomatic sample applicator, Camag TLC scanner 3, Camag Win CATS software, Camag twin trough chamber and a sonicator were used. Tablets containing omeprazole (10 mg) and ondansetron (4 mg) (Ranidom-O, Mankind Pharmaceuticals Pvt. Ltd., Mumbai, India) and capsules containing rabeprazole (20 mg) and ondansetron (6 mg) Omeprazole and rabeprazole (25 mg each) were weighed and diluted with methanol to get the Þnal concentration 0.05 μg/. Μl omeprazole, 0.02 μg/μl ondansetron and 6 μl of standard and sample were applied as 5 mm band on the TLC plate. Samples were applied as bands 5 mm width at 14.1 mm intervals using Camag linomate V semiautomatic sample applicator and migration distance allowed was 80 mm, drying of plate done for 3 min at 600 temperatures.

Spectra comparison

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