Abstract
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F 254. The mobile phase used was a mixture of chloroform: methanol (9:1 v/v). The detection of spots was carried out at 265 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng with regression coefficient of 0.9995. The proposed method can be successfully used to determine the drug content of marketed tablet formulation.
Highlights
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form
EMT and TEN (10 mg each) were weighed accurately, dissolved and diluted with methanol to obtain the final concentration of 0.1 μg/μl of each drug
To carry out HPTLC analysis[6], the TLC plates were prewashed with methanol
Summary
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. An attempt has been made to develop a rapid, accurate, precise and cost effective HPTLC method for simultaneous estimation of EMT and TEN in combined dosage form. EMT and TEN standards were procured as a gift samples from Cipla Pharmaceuticals Ltd. Silica gel 60 F 254 TLC plates
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