Development and validation of a HPTLC method for the estimation of sumatriptan in tablet dosage forms

Cr Shah,Nj Shah,Bn Suhagia,Rr Shah

doi:10.4103/0250-474x.49138

Abstract

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of sumatriptan in tablet dosage forms. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of methanol:water:glacial acetic acid (4.0:8.0:0.1, v/v/v). The detection of spots was carried out at 230 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 800 ng/spot. The limit of detection and the limit of quantification for the sumatriptan were found to be 63.87 and 193.54 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.

Highlights

Sumatriptan standard was procured as a gift sample from Intas Pharmaceuticals Ltd., Ahmedabad
The chromatographic conditions maintained were precoated silica gel 60F254 aluminum sheets (10×10 cm) as stationary phase, methanol:water:glacial acetic acid (4.0:8.0:0.1, v/v/v) as mobile phase, chamber and plate saturation time of 30 min, migration distance allowed was 72 mm, wavelength scanning was done at 230 nm keeping the slit dimension at 5×0.45 mm
Aliquots of 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, and 8.0 μl of standard solution of sumatriptan were applied on the TLC plate (100 μg/ml of drug)

Summary

Introduction

Development and Validation of a HPTLC Method for the Estimation of Sumatriptan in Tablet Dosage Forms Et al.: HPTLC method for the estimation of sumatriptan A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of sumatriptan in tablet dosage forms. The limit of detection and the limit of quantification for the sumatriptan were found to be 63.87 and 193.54 ng/spot, respectively.

Results

Conclusion