Abstract
A spectrofluorimetric method for estimation of mirtazapine in human whole blood was developed and validated. The recovery efficiency of the processing method was 95–98%. The analytical method was linear over drug concentration of 10–200 ng/ml. The limit of quantification was 10 ng/ml. The method was precise with %RSD for intra-day and inter-day precision being <3.0 and 1.5, respectively. Excellent recoveries (97.87–99.69%) were achieved during accuracy studies. The method was robust to small changes in processing method and instrumental parameters. The present method can be employed for direct fluorimetric determination of mirtazapine in human whole blood during clinical studies.
Highlights
Mirtazapine is a piperazinoazepine-based tetracyclic compound which is used as an antidepressant in moderate to severe depression
Numerous sophisticated analytical methods like HPLC [4,5,15,16,17,19,23], LC-MS [2,3,6,7,11,18,25], GC-MS [9,20,22], capillary electrophoresis [13] and enantioselective electrodriven method [14] are available in literature for quantification of mirtazapine in biological fluids, formulations and in the presence of other drugs
A simple, fast and reliable analytical method has always remained a method of choice for quantification of drug in biological matrixes to handle a large number of samples during clinical studies
Summary
Mirtazapine is a piperazinoazepine-based tetracyclic compound which is used as an antidepressant in moderate to severe depression It is classified as an adrenergic and specific serotonergic antidepressant [21,24]. It is 1,2,3,4,10,14b-hexahydro-2-methylpyrazino[2,1-a]pyrido[2,3-c]-2benzazepine which exists as a mixture of (−) and (+) enantiomers (Fig. 1) and both have similar pharmacological activity [1]. It acts by facilitating central serotonergic and nor-adrenergic transmission and antagonizes postsynaptic 5-HT2A, 5-HT3 and H1 receptors [8]. There is no report of any spectrophometric or spectrofluorimetric method for quantification of mirtazapine in human whole blood
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