A study of protein and glycoprotein syntheses in pre-implantation mouse embryos using mini-2D-electrophoresis, video densitometer scanning and computer-image analysis.

Abstract

In the present study, proteins and glycoproteins of mouse embryos at 2-cell, morula and blastocyst stages were analyzed. The techniques of 35S-Met incorporation, ConA antiserum-precipitating ConA-binding proteins, mini-2D-electrophoresis, fluorography, video densitometer scanning and the computer-image system were used for analyses. Results of the investigations indicated that proteins and glycoproteins were synthesized by the embryos in a gradual increase manner from 2-cell to blastocyst. A relatively large amount of glycoproteins was synthesized during the morula and blastocyst stages. Two specific small glycoproteins respectively with molecular weights about 6500 and 9000 and PIs at 5.0 and 6.5 were apparently synthesized in the blastocyst but not in the 2-cell or the morula.