Abstract
The direct chemical interaction of catecholamines with adenine nucleotides has been examined using the technique of nuclear magnetic resonance spectroscopy. Preferential stabilization of the alpha and beta carbons of the side chain of epinephrine has been demonstrated by selective changes in the relaxation times of the protons attached to these carbons. By varying the concentrations of amine and nucleotide, it has been shown that at pH 5.6, three molecules of epinephrine can interact with one molecule of ATP and one molecule of epinephrine can interact with one molecule of AMP. An additional catecholamine molecule may be attached to the adenine nucleotide complexes at a higher pH. The complex appears to involve an ionic bond between the catecholamine nitrogen and the phosphate moiety of the nucleotide and a hydrogen bond between the beta hydroxyl group of the catecholamine and a phosphate oxygen. The very small preferential stabilization of the side chain carbons of dopamine in the presence of ATP indicates that the beta hydroxyl group of the catecholamine plays a role in the interaction.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Naunyn-Schmiedebergs Archiv fur experimentelle Pathologie und Pharmakologie
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
