A Stretching Force Control-Based Cyclic Loading Method for the Evaluation of Mechanical Properties of Gelation Methacrylate (GelMA) Microfibers.

Abstract

Microfluidic spun gelation mechacrylate (GelMA) microfiber has been widely utilized as a promising bioink for 3D bioprinting. However, its weak and easily tuned mechanical properties are still difficult to precisely evaluate, due to the lack of an effective stretching method. In this paper, we propose a force-control-based cyclic loading method for rapidly evaluating the elastic modulus: the E of the microfibers with different GelMA concentrations. A two-tube manipulation system is used to stretch microfiber with a non-destructive process. Based on the model reference adaptive control strategy, the stress response can be fitted into a sinusoidal wave when a small sinusoidal strain is automatically applied onto the microfiber. Afterwards, the maximum tensile stress and tensile stain is obtained to determine the E. Moreover, different stress amplitudes and frequencies are applied to form different stress-strain loops with almost same E. Compared with a frequently-used constant force loading method, the proposed method shows an obvious advantage in measurement accuracy, especially for low-concentration GelMA microfiber. Furthermore, the reasonableness of the measured E for different GelMA concentrations is confirmed by 3D cell culture experiments, and the results show the proposed method has great application potential to investigate the interaction between cell and fibrous bioink substrate.