A Sensitive Electrochemical Sensor for Rapid Determination of Mebeverine Hydrochloride and Metronidazole Benzoate Selective Molecular Imprinted Polymer in the PVC Membrane

Inas Hasan Mohammed Al Khafaji,Yehya Kamal Khaleel Al-Bayati

doi:10.30526/31.1.1855

Inas Hasan Mohammed Al Khafaji, Yehya Kamal Khaleel Al-Bayati

Open Access

https://doi.org/10.30526/31.1.1855

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Abstract

By using precipitation polymerization, liquid electrodes of polymers imprinted with Mebeverine hydrochloride and metronidazole benzoate were created whereas the imprinted polymer (MIP) and non imprinted (NIP) polymers were prepared by using Mebeverine hydrochloride and Metronidazole benzoate qua a template. In the polymerization process, 2-Acrylamido-2-methyl-1-propane Sulphonic acid (AMPS) or 1-Vinylimidazole (VIZ) was used qua monomer, pentaerythritol triacrylate (PETRA) or Divinylbanzene (DVB) was used qua a cross-linker while benzoyl peroxide (BPO) was used as an initiator. The MIP membranes and the membranes of NIP were created by using Dibutyl Sebacate (DBS) and Tris(2-ethylhexyl)phosphate(TEHP) qua plasticizers in PVC matrix. The response time of the liquid electrodes was 1min. whereas their slopes and detection limits reached to 19.62 â€“ 57.36 mV per decade and 1.2 x 10-6 â€“ 2.0 x 10-5 M, respectively. Filling with standard solution of drug (0.1M), the liquid electrodes response -with suitable No.( selectivity for numerous of species - was suitable No.( since pH reached to 1.5 â€“ 12. The developed electrodes were successfully applied for the analyte determination in preparation pharmaceutical sample without any time consuming pretreatment steps.

Highlights

As one of the nitroimidazole derivative, Metronidazole benzoate(MNZB), figure (1)1-(2benzyloxy ethyl)-5-nitro methylimidazole is recognized for its antimicrobial features as this compound is effective before trichomonas, anaerobic bacteria and Vincent’s organisms
Liquid electrode membranes The working ranges of liquid electrode membranes, Nernstian response and their slopes have been studied depending on molecularly imprinted electrodes sensors (MIP) liquid electrodes which is made of the monomers AMPS and VIZ combined in a PVC matrix plus the two plasticizers DBS and TEHP
The findings show that the two monomers can be used for producing effective MIPs for Mebeverine hydrochloride (MBV) and MNZB in spite of that the acid-base properties of them are different

Summary

Introduction

As one of the nitroimidazole derivative, Metronidazole benzoate(MNZB), figure (1)1-(2benzyloxy ethyl)-5-nitro methylimidazole is recognized for its antimicrobial features as this compound is effective before trichomonas, anaerobic bacteria and Vincent’s organisms. The performance of the electrode was investigated by measuring the potential of MBV.HCl and Metronidazole benzoate solutions at room temperature with a concentrations range from10-6 to 10-1M. The calibration curves obtained by plotted the response against logarithmic function of mebeverine and Metronidazole benzoate concentration.

Results

Conclusion