Abstract
An assay has been developed for deoxyribonucleoside triphosphates; it is based on the observation that all four nucleotides are necessary for the continued synthesis of DNA. A linear standard curve is obtained with one of the nucleotides as a limiting factor and measurement of the amount of incorporation into the polymer of a radioactive deoxyribonucleoside triphosphate present in excess. The method has been utilized to determine the concentration of dTTP in HClO 4 extracts of mouse leukemia cells and Streptococcus faecalis. Other materials present in the extracts did not influence the assay. The method offers the advantages of simplicity, speed, and sensitivity.
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