Abstract
A biosensor was developed for using in a Lab-On-a-Chip (LOC). The sensor detects the change in the resonance frequency of a micro-cantilever with a piezoelectric film. This is the mass micro-balancing technique, which has been successfully used for detecting bio-materials in the quartz crystal microbalance (QCM). The PZT film, a piezoelectric film, is designed to act as both sensor and actuator. The geometry of the micro cantilever is optimized to maximize the sensitivity and minimize the environmental effects such as viscous damping and added mass effect in liquid. The fabricated sensor is composed of a 100 μm long, 30 μm wide, and 5 μm thick cantilever with a 2.5 μm thick piezoelectric (PZT) layer on it. The ratio of thickness to length of the micro cantilever is very high compared to others in micro cantilever-based studies. This high aspect ratio is the key to maximize the sensitivity and minimize the environmental effects. The fabricated micro sensor was tested by detecting the mussel gluing protein, the insulin-anti insulin binding protein and the poly T-sequence DNA.
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