Abstract
Garlic mustard oil macerate is a traditional medicine used as a nasal decongestant by the people of northeast India. In this study, a unique preparation technique that maximized the yield of organosulfur compounds in GMM was developed at the lowest preparation temperature and time duration compared to existing preparation methods. Liquid chromatography-mass spectrometry (LCMS), central composite design (CCD) and high-performance liquid chromatography (HPLC) were used for compound identification, experimental design, and quantification of the organosulfur compounds (OSCs), respectively. Through LCMS analysis, ajoene and 2-vinyl-4H-1,3-dithiin were detected as the major OSCs. CCD analysis suggested 17 experiments for HPLC analysis, after which the quantity of mustard oil and heating temperature were found to be the significant parameters for optimum OSCs formation. Based on this, the optimized conditions to maximize the yield of ajoene ((garlic: oil (1:2.00), 55.00°C, 4 1 2 h)) and 2-vinyl-4H-1,3-dithiin ((garlic: oil (1:2.20), 77.51°C, 2 1 2 h)) were achieved.
Published Version
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