Abstract
Simple SummaryMany Chinese-local chickens show slow-growing and low-producing performance, which is not conductive to the development of the poultry industry. The identification of thousands of indels in the last twenty years has helped us to make progress in animal genetics and breeding. Golgin subfamily B member 1 (GOLGB1) is located on chromosome 1 in chickens. Previous study showed that a large number of QTLs on the chicken chromosome 1 were related to the important economic traits. However, the biological function of GOLGB1 gene in chickens is still unclear. In this study, we detected a novel 65-bp indel in the fifth intron of the chicken GOLGB1 gene. Correlation analysis between the 65-bp indel and chicken growth and carcass traits was performed through a yellow chicken population, which is commercial. Results revealed that this 65-bp indel was significantly associated with chicken body weight, highly significantly associated with neck weight, abdominal fat weight, abdominal fat percentage, and the yellow index b of breast. These findings hinted that the 65-bp indel in GOLGB1 could be assigned to a molecular marker in chicken breeding and enhance production in the chicken industry.Golgin subfamily B member 1 (GOLGB1) gene encodes the coat protein 1 vesicle inhibiting factor, giantin. Previous study showed that mutations of the GOLGB1 gene are associated with dozens of human developmental disorders and diseases. However, the biological function of GOLGB1 gene in chicken is still unclear. In this study, we detected a novel 65-bp insertion/deletion (indel) polymorphism in the chicken GOLGB1 intron 5. Association of this indel with chicken growth and carcass traits was analyzed in a yellow chicken population. Results showed that this 65-bp indel was significantly associated with chicken body weight (p < 0.05), highly significantly associated with neck weight, abdominal fat weight, abdominal fat percentage and the yellow index b of breast (p < 0.01). Analysis of genetic parameters indicated that “I” was the predominant allele. Except for the yellow index b of breast, II genotype individuals had the best growth characteristics, by comparison with the ID genotype and DD genotype individuals. Moreover, the mRNA expression of GOLGB1 was detected in the liver tissue of chicken with different GOLGB1 genotypes, where the DD genotype displayed high expression levels. These findings hinted that the 65-bp indel in GOLGB1 could be assigned to a molecular marker in chicken breeding and enhance production in the chicken industry.
Highlights
Golgin subfamily B member 1 (GOLGB1) gene is a widely expressed large coiled-coil protein, and a Golgi-associated large transmembrane protein [1]
We further studied the relationship between the 65-bp indel in GOLGB1 and chicken growth traits, and further observed its expression patterns in different tissues
The mRNA expression level of the DD genotype was significantly higher than in the II and ID genotypes (p < 0.01, Figure 4). These results suggested that the different genotypes might have different effects on chickens
Summary
Golgin subfamily B member 1 (GOLGB1) gene is a widely expressed large coiled-coil protein, and a Golgi-associated large transmembrane protein [1]. A previous study reported that the GOLGB1 loss-of-function mutation was co-segregated with cleft palate, and GOLGB1 mutant embryos showed intrinsic defects in palatal shelf elevation. These results suggest that GOLGB1 plays an important role in glycosylation and tissue morphogenesis of protein [4]. Another previous study reported that GOLGB1 gene is expressed in cultured chondrocytes, and various organs and embryos during different developmental stages [5]. A genome-wide associated study has shown that one mutation in GOLGB1 (Y1212C)
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