Abstract
Non-thermal biocompatible plasma (NBP) was considered as an efficient tool in tissue engineering to modify the surface of biomaterials. Three-dimensional chitosan scaffolds have been extensively used in different ways because it holds some remarkable properties, including biodegradability and biocompatibility. In this study, we evaluated the osteogenic potential of NBP-treated chitosan scaffolds using two different plasma sources: a dielectric barrier discharge (NBP-DBD) and a soft jet (NBP-J). The surface modification of the scaffold was evaluated using scanning electron microscopy. For osteogenic differentiation of cells, proliferation and differentiation were tested by using bone marrow-derived stem cells (BMSCs). We observed that cell viability using NBP-DBD and NBP-J treated chitosan scaffolds yielded significant improvements in cell viability and differentiation. The results obtained with MTT and live/dead assays showed that NBP-modified scaffold increases cell metabolic by MTT assay and live/dead assay. It also observed that the NBP treatment is more effective at 5 min with DBD and was selected for further investigations. Enhanced osteogenic differentiation was observed using NBP-treated scaffolds, as reflected by increased alkaline phosphatase activity. Our findings showed that NBP is an innovative and beneficial tool for modifying chitosan scaffolds to increase their activity, making them suitable as biocompatible materials and for bone tissue engineering.
Highlights
Non-thermal biocompatible plasma (NBP) is a powerful tool for the development of innovative approaches in biological and medical fields [1]
Our findings showed that NBP is an innovative and beneficial tool for modifying chitosan scaffolds to increase their activity, making them suitable as biocompatible materials and for bone tissue engineering
Lauriault et al reported that NBP induces micropatterned nitrogen-rich deposition on the surface of a polymer film with enrichment of microphages, chondrocyte adhesion, and proliferation, which can be used for tissue engineering [21]. These results indicated that NBP is an advantageous tool for surface modification of biomaterials used in tissue engineering
Summary
Non-thermal biocompatible plasma (NBP) is a powerful tool for the development of innovative approaches in biological and medical fields [1]. NBP can be generated using various ionizing gases. NBP comprises many charged particles, including ions and electrons, reactive oxygen species (ROS), reactive nitrogen species (RNS) (or reactive oxygen/nitrogen species [RONS]), ultraviolet light, as well as electric and magnetic fields [2]. RONS generated by plasma play a significant role in various biological functions and have several applications, including cancer therapy, wound healing, dental care, skin diseases, and sterilization [1–10]. NBP interacts with stem cells to enhance their differentiation [11–15]. NBP has been used extensively for surface modification of biomaterials [16–18]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call