Abstract
Simple SummaryIn the last few years, multiple new infectious diseases have affected amphibians, causing unprecedented declines and extinctions at the global scale. Many of these diseases are caused by pathogens that have been described during recent decades. In this study we report a novel disease, affecting European amphibians. In a protected area of Northern Italy, since the autumn of 2013, we started to find some adult salamanders with cysts at the throat level (subgular region). Following this observation, we ran a regular monitoring of the salamander population and performed multiple morphological and molecular investigations to identify the cause of this undescribed disease. The cysts surround peculiar cells, probably protists, of about 30 µm covered by numerous motile cilia/undulipodia. Despite multiple attempts with a broad spectrum of techniques, the detailed identification remains challenging as we have been unable to match its features with previously described organisms. We provide the results achieved till now to promote a rapid dissemination on this new enigmatic wildlife pathogen and to create a basis for further and deeper studies.New pathologies are causing dramatic declines and extinctions of multiple amphibian species. In 2013, in one fire salamander population of Northern Italy, we found individuals with undescribed cysts at the throat level, a malady whose existence has not previously been reported in amphibians. With the aim of describing this novel disease, we performed repeated field surveys to assess the frequency of affected salamanders from 2014 to 2020, and integrated morphological, histological, and molecular analyses to identify the pathogen. The novel disease affected up to 22% of salamanders of the study population and started spreading to nearby populations. Cysts are formed by mucus surrounding protist-like cells about 30 µm long, characterized by numerous cilia/undulipodia. Morphological and genetic analyses did not yield a clear match with described organisms. The existence of this pathogen calls for the implementation of biosecurity protocols and more studies on the dynamics of transmission and the impact on wild populations.
Highlights
IntroductionEpigraph “Нo кaк же этo тaк? Bедь этo же чудoвищнo! Этo чудoвищнo, гoспoдa”,-пoвтoрил oн, oбрaщaясь к жaбaм в террaрии, нo жaбы спaли и ничегo ему не oтветили.” (“But how can it be? It’s monstrous! Quite monstrous, gentlemen,”he repeated, addressing the toads in the terrarium, who were asleep and made no reply)
The fire salamander is ovoviviparous and widespread in Europe; this species mainly inhabits hilly landscapes covered by broadleaf forests and breeds in streams and other freshwater sites [26]
The area is characterized by an extended broadleaf wood crossed by a slow flowing stream and numerous springs where the fire salamander breeds
Summary
Epigraph “Нo кaк же этo тaк? Bедь этo же чудoвищнo! Этo чудoвищнo, гoспoдa”,-пoвтoрил oн, oбрaщaясь к жaбaм в террaрии, нo жaбы спaли и ничегo ему не oтветили.” (“But how can it be? It’s monstrous! Quite monstrous, gentlemen,”he repeated, addressing the toads in the terrarium, who were asleep and made no reply). He repeated, addressing the toads in the terrarium, who were asleep and made no reply). The emergence and spreading of novel diseases in wildlife is one of the most challenging threats to both biodiversity and human well-being [1–4]. When diseases emerge in wildlife organisms, their detection is not easy and some pathogens may remain unnoticed till their spreading becomes difficult to control [8,9]. This could be true for elusive or poorly studied animals, as ‘non-mammals’ vertebrates, and when pathologies are strongly different from the already known diseases
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