Abstract
With the worldwide emergence of health challenges facing humanity in the form of associated immunity and intestinal health, the utilization of effective probiotics is becoming prevalent. We developed a sustainable, biocompatible, rigid, and long-lasting enteric coating system using pH-responsive natural-based materials, shellac (Sh), and cellulose nanocrystals (CNC) which could be utilized as a carrier material for intestinal delivery. As a proof-of-concept, the yeast ( Saccharomyces cerevisiae ) as a model, was encapsulated in the ShCNC microcapsules with CaCl 2 (Ca). We elucidated the 3D structure of ShCNCCa microcapsules using a confocal laser microscope. A 1–1.3 μm shell consisting of shellac and CNC nanocomplex protected the yeast resulting in high viability and retention of 602.4% under harsh biological conditions. During processing, storage and transport through the gastric environment, the yeasts were protected and then triggered to release in the intestinal environment of pH 7–8. Viscosity synergism and mucoadhesion analysis revealed that the microcapsules bound strongly to the mucus membrane. • The W/O Pickering emulsion technique produced uniform microcapsules containing S. cerevisiae. • The microcapsules maintained the survivability of S. cerevisiae in gastric environments. • 1–1.3 μm shell consisting of ShCNCCa nanocomplex protected the yeast resulting in high retention. • The mucoadhesion demonstrated that microcapsules adhere to the mucus membrane.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call