Abstract
Phospholipid synthesis in the yeast Saccharomyces cerevisiae is a complex process that involves regulation by both genetic and biochemical mechanisms. The activity levels of phospholipid synthesis enzymes are controlled by gene expression (e.g., transcription) and by factors (lipids, water-soluble phospholipid precursors and products, and covalent modification of phosphorylation) that modulate catalysis. Phosphatidic acid, whose levels are controlled by the biochemical regulation of key phospholipid synthesis enzymes, plays a central role in the regulation of phospholipid synthesis gene expression.
Highlights
Phospholipid synthesis in the yeast Saccharomyces cerevisiae is a complex process that involves regulation by both genetic and biochemical mechanisms
We focus on how genetic and biochemical mechanisms work together to regulate phospholipid synthesis
We focus on phospholipid synthesis genes that contain the inositolresponsive element (UASINO) and that are regulated by the transcription factors Ino2, Ino4, and Opi1
Summary
Mutants (e.g., cho, psd psd, cho, and opi3) defective in the CDP-DAG pathway can synthesize PE or PC if they are supplemented with ethanolamine, choline, lysoPE, lysoPC, or PC with short acyl chains [1, 20,21,22,23]. Kennedy pathway mutants (e.g., cki eki and cpt ept1) defective in both the CDP-choline and CDPethanolamine branches synthesize PC only via the CDPDAG pathway [19, 30, 31] These mutants, unlike those defective in the CDP-DAG pathway [1], do not exhibit any auxotrophic requirements [19, 31]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
