Abstract
A phenol—water—ammonia solvent system has been utilized to separate most of the known phospholipids and lysophospholipids on thin-layer plates of silica gel G. These compounds were identified by chromatography of their mild alkaline hydrolysis products, various staining reactions, and comparison with known compounds. An unknown radioactive phospholipid has been detected in pigeon pancreas lipids by means of this chromatographic method. A procedure is described which makes it possible to determine the radioactivity incorporated into the individual phospholipids with a high degree of precision.
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