Separation and fluorimetric determination of primary alkylamines by reversed-phase thin-layer chromatography after derivatization into 3-alkyl-2,2-diphenyl-1-oxa-3-azonia-2-boratanaphthalenes.

Abstract

The “reversed-phase” thin-layer chromatography (plates:silica gel 60 silanized, mobile phase:methanol/water, 80:20, v/v) is more suited for the separation of primary n-alkylamines after their derivatization into 3-alkyl-2, 2-diphenyl-1-oxa-3-azonia-2-boratanaphthalenes than the method using thin-layer plates of silica gel 60 and benzene/chloroform (80:20, v/v). The quantitative analysis of amine mixtures is achieved by in situ fluorimetry of the developed chromatogram. With a limit of detection of 30 ng/8 μl for n-hexylamine, this method allows the quantitative determination of, e.g., 162 ng in 8 μl sample volume with a standard deviation of ± 7 ng hexylamine. The separation and quantitative analysis of decylamine from river water, containing amines, is described here.