Abstract
A method for producing simultaneously homogeneous preparations of two main forms of metleghemoglobin reductase and major leghemoglobin components from lupine nodules was developed. The method involves salting out with ammonium sulfate at 40–80% saturation, gel filtration on Ultrogel AcA 44, and double isoelectric focusing. The homogeneous metleghemoglobin reductase forms with molecular weights of 62 and 66 kDa were purified 725- and 402-fold, respectively. The total yield with respect to activity equaled 37%. The 62-kDa form was more active.
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