Abstract
Compared to classical ‘forward’ genetics in which the changed phenotype of an organism is correlated with mutations in the genome, the availability of complete nucleotide sequences of many microbes and animals now allows the rational engineering of mutations in individual genes and identification of the correlated change in phenotype of the reconstituted organism (‘reverse genetics’)
Highlights
Rotaviruses (RVs) are a major cause of acute gastroenteritis (AGE) in infants and young children worldwide and in many mammalian and avian species[1,2]
Based on RV structure and classification, the viral replication cycle and viral genetics, the recent achievement of a plasmid only-based reverse genetics (RG) for RV enabling the recovery of infectious virus from cDNA which can be mutated precisely is briefly described and put into the larger context of RV research
Rotavirus mutants can be studied by complementation or reassortment analyses, e.g. for most of the temperature-sensitive RV mutants genetic mutations have been localized to individual RNA segments[9]
Summary
Rotaviruses (RVs) are a major cause of acute gastroenteritis (AGE) in infants and young children worldwide and in many mammalian and avian species[1,2]. RV-associated disease led to the death of over 200,000 children of
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