A green-micro-synthesis of curcumin functionalized silver nanoparticles for bacteria inhibition and glucose sensor electrode modifier

Abstract

A novel strategy for silver nanoparticles synthesis has been developed in a simple way. The method promotes green synthesis by using demineralized water as the primary solvent, sunlight as the energy source, and the small volume of the reaction. The silver nanoparticles were obtained by utilizing the autooxidation pathways of curcumin that was dissolved in ethanol. Interestingly, the volume of curcumin at the microliter level could produce AgNPs with good properties. The materials were characterized using UV–Visible spectrophotometer, Fourier Transform Infra-Red Spectroscopy (FTIR), Scanning Electron Microscope (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Spectroscopy (EDX), and Nano Particle Analyzer. The bacterial inhibition activity of silver nanoparticles (cAgNPs) was tested against Eschericia coli ATRCC6633 and Staphylococcus aureus ATRCC6633. This material showed good activity in inhibiting Gram-negative and Gram-positive bacteria compared to the control Ag + ion and curcumin. MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) test results exhibited a good performance of cAgNPs against E. coli ATRCC6633 and S. aureus ATRCC6633. Carbon paste electrode (CPE) modified with cAgNPs was used to detect glucose using Differential Pulse Voltammetry (DPV) technique. The cAgNPs were electrodeposited on CPE using cyclic voltammetry (CV) technique. The modified electrode showed promising results to be applied for glucose detection due to its good linearity and sensitivity. In addition, this work will give new insight into a sustainable method for producing silver nanomaterials that provide good bacteria inhibition and enhance the CPE performance to detect glucose.