Abstract
In this work, a novel colorimetric and ratiometric fluorescent probe (R1) for detecting Hg2+ based on xanthene and naphthimide groups via the fluorescence resonance energy transfer (FRET) mechanism was designed and synthesized. In the CH3CN/H2O (1/1, v/v) system, the probe R1 showed ratiometric fluorescent responsiveness to Hg2+ with the characteristics of rapid response time (less than 30 s), high selectivity and excellent anti-interference ability, meanwhile, the fluorescence color changed from cyan to orange and the solution color changed from colorless to pink by naked-eye. Based on fluorescence and UV/vis measurements, the limit of detection (LOD) was calculated to be 1.14 μM and 0.17 μM, respectively. Furthermore, the possible mechanism for the detection of Hg2+ of probe R1 was verified by using ESI-MS, 1H NMR and HPLC spectra.
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