Abstract
In this study, a convenient assay method has been developed based on labeled functional nucleic acids (H-DNA) and a competitive fluorescent lateral flow immunoassay (CF-LFI) for ampicillin (AMP) detection. Herein, we designed the tunable AMP probes for AMP detection based on the AMP aptamer, and the secondary DNA fragment. The probes can generate tunable signals on the test line (T line) and control line (C line) according to the concentration of AMP. The accuracy of detection was improved by optimizing the tunable AMP probes. Under the optimal conditions, the linear concentration of AMP detection is ranged from 10 to 200 ng/L with a limit of quantitation (LOQ) value of 2.71 ng/L, and the recovery is higher than 80.5 %. Moreover, the developed method shows the potential application for AMP detection in the hospital wastewater.
Highlights
Ampicillin (AMP), a beta-lactam antibiotic, has been widely used in the medical, aquaculture, and agriculture-based industries to prevent and treat bacterial infections of Haemophilus influenzae, Escherichia coli, and Salmonella as well as Shigella species [1,2]
Several on-site tests have been recognized as promising approaches for antibiotic detection owing to specific advantages of low cost and convenience
These antibiotic assays conducted by chemiluminescence [4], fluorescence (FL), electrochemistry, and chromatography exhibited numerous benefits in diverse applications [5,6]
Summary
Ampicillin (AMP), a beta-lactam antibiotic, has been widely used in the medical, aquaculture, and agriculture-based industries to prevent and treat bacterial infections of Haemophilus influenzae, Escherichia coli, and Salmonella as well as Shigella species [1,2]. Several on-site tests have been recognized as promising approaches for antibiotic detection owing to specific advantages of low cost and convenience These antibiotic assays conducted by chemiluminescence [4], fluorescence (FL), electrochemistry, and chromatography exhibited numerous benefits in diverse applications [5,6]. To meet the demand of rapid detection and high sensitivity, LFI has garnered increasing interest in recent times for the substantial detection of antibiotic residues in water. Chen and his coworkers developed the near-infrared (NIR) FL-based LFI for simultaneous detection of four antibiotic residues and improved the sensitivity [7]. It is of significance to develop a highly-sensitive, selective, simple, and fast detection for AMP assay in the wastewater
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