Abstract
Neurospora mtDNA contains a repetitive, 18 nucleotide palindromic sequence (5'-CCCTGCAGTACTGCAGGG-3') that contains two closely spaced PstI sites (CTGCAG) in the arms of the palindrome (Yin, S., Heckman, J., and RajBhandary, U. L. (1981) Cell 26, 325-332). In the present study, DNA sequence analysis was carried out to determine whether PstI palindromes are present in an apparently distinct genetic element, the 3.6-kilobase mitochondrial plasmid from Neurospora crassa strain Mauriceville-1c (FGSC 2225). The plasmid contains a cluster of closely spaced PstI sites extending over a 0.4-kilobase region (Collins, R. A., Stohl, L. L., Cole, M. D., and Lambowitz, A. M. (1981) Cell 24, 443-452). The DNA sequence shows that the cluster consists of eight PstI sites organized in five palindromic elements. Two of the elements are identical with the canonical sequence found in mtDNA, whereas the remaining three elements differ from the canonical sequence by a few nucleotides. The occurrence of the PstI palindromes in two otherwise unrelated DNA species is consistent with the hypothesis that they are related to mobile DNA sequences that either propagate or were once capable of propagating within mitochondria.
Highlights
The DNA sequence shows that the cluster consists of eight PstI sites organized in five palindromic elements
DNA sequence analysis shows that the latter cluster contains eight PstI sites organized in five palindromic elements
Two pairs ofPstI s4i5te4s, beginning at positions 107 and 454, are present in the canonical 18-nucleotidesequence, 5'CCCTGCAGTACTGCAGGG-3' (Fig. 2, a and e). Another pair of PstI sites is found in an 18-nucleotidesequence which begins at nucleotide 323 and which differsfrom the canonical sequenceat the 2nd and 17th positions
Summary
DNA sequence analysis was gene new opposite ends of the 2.3-kilobase intervening secarried out to determine whether PstI palindromes are quence (Yin et al.,1981).The yeast GC-rich clusters are most present in an apparently distinct genetic element, the often located outside of genes, but aresometimes found within The DNA sequence shows that the cluster consists of eight PstI sites organized in five palindromic elements.
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