Abstract
An electrochemical screen-printed paper based analytical device (EµPAD) was fabricated for the detection of cholesterol based on two electrode system. The working electrode of EµPAD was coated with graphene oxide (GO) and polyaniline composite. The GO was chemically synthesized in the laboratory using modified hummer’s method and characterized using XRD, Raman, UV – Visible absorption, FTIR spectroscopies, and SEM studies. Cholesterol oxidase (ChOx) enzyme was immobilized on the GO/PANI modified EµPAD for the electrochemical detection of cholesterol using cyclic voltammetry (CV) and chronoamperometry. In both measurements, the current increased when the concentration of cholesterol increases from 1 mM to 10 mM. This increase in current with increase in cholesterol concentration was due to fact that more H2O2 molecules were generated when the cholesterol concentration was increased. Further, H2O2 is oxidized and electrons were generated which enhanced the current.
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