Abstract
A method for culturing and cloning a cell line derived from rat hepatic tissue is described. The cloned cells in culture were diploid, epithelioid in morphology, and synthesized tyrosine aminotransferase. The cytotoxic effects of the hepatocarcinogen aflatoxin B1 on these cells were compared to the effects on an established cell culture. The diploid culture was found to be more resistant to the toxin as measured by cell counts and residual protein as well as by [14C]uridine incorporation.
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