Abstract
Postnatal rat heart cells in culture enriched with respect to muscle cells were obtained by either high density seeding or by the replating technique. [3H]Thymidine incorporation to DNA and the enzymatic pattern of cytoplasmic and lysosomal enzymes have been studied as a function of the culture's age, of seeding density, and replating. It was shown that replating maintains predominance of myocyte population for at least 2 wk in culture; heavy seeding density allows homogeneous myocyte population for the 1st wk in culture; and the enzyme profile of the culture may serve as an indicator for the type of cell population in culture and its state of differentiation.
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