A compartmental model of zinc metabolism in adult men used to study effects of three levels of dietary copper

Abstract

The effect of dietary copper level on zinc metabolism in five healthy adult men was studied using compartmental modeling. The subjects were fed one level of dietary zinc (11.2 +/- 1.6 mg/day) and three levels of dietary copper (1.68, 0.785, and 7.52 mg/day). The stable isotope tracers 70Zn and 67Zn were administered intravenously (3 times) and orally (4 times), respectively. Plasma, urinary, and fecal 67Zn and 70Zn levels were measured. An existing model of zinc metabolism was used as a basis for our model. Model rate constants were modified to simulate multiple oral and intravenous doses of stable isotope tracers given during the 90-day study. Most rate constant changes occurred in the absorption and excretion pathways. The model predicted that movement of zinc through the gastrointestinal tract increased when the highest level of copper was fed and the urinary zinc excretion rate tended to increase. The fractional rate constant representing absorption of zinc decreased slightly when the highest level of copper was fed. Total body zinc was predicted to be approximately 2,000 mg, with 95% in tissues other than the liver, red blood cells, or plasma and approximately 2.25% in the blood (with 95% of that in the red blood cells), which follows expected zinc masses within the body and those predicted by previous models.