Extensive Literature Search on the ‘Effects of Copper intake levels in the gut microbiota profile of target animals, in particular piglets’

Abstract

EFSA Supporting PublicationsVolume 13, Issue 5 1024E External scientific reportOpen Access Extensive Literature Search on the ‘Effects of Copper intake levels in the gut microbiota profile of target animals, in particular piglets’ Bent Borg Jensen, Bent Borg JensenSearch for more papers by this author Bent Borg Jensen, Bent Borg JensenSearch for more papers by this author First published: 02 May 2016 https://doi.org/10.2903/sp.efsa.2016.EN-1024Citations: 3 The present document has been produced and adopted by the bodies identified above as author(s). This task has been carried out exclusively by the author(s) in the context of a contract between the European Food Safety Authority and the author(s), awarded following a tender procedure. The present document is published complying with the transparency principle to which the Authority is subject. It may not be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors. Published date: 2 May 2016 Question number: EFSA-Q-2015-00680 AboutPDF ToolsExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Alphabetic list of references included in the ELS for pigs and piglets (* ID number according to Annex A1) Armstrong TA, Williams CM, Spears JW and Schiffman SS, 2000. High dietary copper improves odor characteristics or swine waste. Journal of Animal Science 78, 859– 864. P140* Armstrong TA, Cook DR, Ward MM, Williams CM and Spears JE, 2004. Effect of dietary source (cupric citrate and cupric sulphate) and concentration on growth performance and fecal copper excretion in weanling pigs. Journal of Animal Science 82, 1234– 1240. P141* Aviotti MP, deLima FR, Langlois BE, Stahly TS and Cromwell GL, 1980. Effect of single additions and combinations of copper and antibiotics on the performance and fecal coliform resistance patterns of swine. Journal of Animal Science 51 Suppl. 1, 184– 185. P5* Bunch RJ, Speer VC, Hays VW, Hawbaker, JH and Carton DV, 1961. Effects of copper sulfate copper oxide and chlortetracycline on baby pig performance. Journal of Animal Science 20, 723– 726. P142* Dierick NA, Vervaeke IJ, Decuypere JA and Henderickx HK, 1986. Influence of the gut flora and some growth-promoting feed additives on nitrogen-metabolism in pigs. 1. Studies in vitro. Livestock Production Science 14, 161– 176. P 19* Fuller R, Newland LGM, Briggs CAE, Braude R and Mitchell KG, 1960. The normal intestinal flora of the pig. IV. The effect of dietary supplements of penicillin, chlortetracycline or copper sulphate on the fecal flora. Journal of Applied Bacteriology 23, 195– 205. P143* Hawbaker JA, Speer VC, Hays VW and Carton DV, 1961. Effect of copper sulfate and other chemotherapeutics in growing swine rations. Journal of Animal Science 20, 163– 167. P144* Henderickx HK, Vervaeke IJ, Decuypere JA and Dierick NA, 1982. Effect of growth promoting agents on the intestinal gut flora. Advanced Veterinary Medicine 33, 56– 62. P145* Hu CH, Xia MS, Xu ZR and Xiong L, 2004. Effect of copper-bearing montmorillonite on growth performance and digestive function of growing pigs. Asian-Austraiasian Journal of Animal Science 17, 1575– 1581. P46* Huang Y, Zhou TX, Lee JH, Jang HD, Park JC and Kim IH, 2010. Effect of dietary copper sources (Cupric sulfate and cupric methionate) and concentrations on performance and fecal characteristics in growing pigs. Asian-Austraiasian Journal of Animal Science 23, 757– 761. P146* Hojberg O, Canibe N, Poulsen HD and Jensen BB, 2005. Influence of dietary zinc oxide and copper sulfate on the gastrointestinal ecosystem in newly weaned piglets. Applied and Environmental Microbiology 71, 2267– 2277. P41* Jensen BB, 1998. The impact of feed additives on the microbiology of the gut in young pigs. Journal of Animal Feed Science 7, 45– 64. P49* Kellogg TF, Hays VW, Catron DV, Quinn LY and Speer VC, 1966. Effect of dietary chemotherapeutics on the performance and fecal flora of baby pigs. Journal of Animal Science 25, 1102– 1106. P147* Kroger H, Feder H and Plischke R, 1977. Untersuchungen uber den Einfluss verschiedener Cu-Zusatze (Kupfersulfat, Kupfer-II-Oxid und elementares Kupfer) auf die Mast- und Schlachteistungen beim Schwein. Zuchiungskunde 49, 213– 214. P59* (In German) Li R, Lu Y, Hu E-y, Zi Z-h, Sun, G-r and Xia, 2014. Effect of copper glycinate on the fecal copper content and bacterial flora of weanling pig. Acta Agricuiturae Shanghai 30, 15– 18. P148* (In Chinese) Ma YL, Guo T and Xu ZR, 2007. Effect of Cu (II)-exchange montmorillonite on diarrhea incidence, intestinal microflora and mucosa morphology of weaning pigs. Chinese Journal of Veterinary Science 27, 279– 283. P69* (In Chinese) Mei SF, Yu B, Ju CF, Zhu D and Chen DW, 2009. Effect of high levels of dietary zinc and copper on growth performance, digestive physiology and cecal microbe in weanling piglets. Chinese Journal of Animat Nutrition 21, 903– 909. P74* (In Chinese) Mei SF, Yu B, Ju CF, Zhu D and Chen DW, 2010. Effect of different levels of copper on growth performance and cecal ecosystem of newly weaned piglets. Italian Journal of Animal Science 9, 378– 381. P72* Namkung H, Gong H, Yu H and deLange CFM, 2006. Effect of pharmacological intakes of zinc and copper on growth performance, circulating cytokines and gut microbiota of newly weaned piglets, challenged with coliform lipopolysaccharides. Canadian Journal of Animal Science 86, 511– 522. P 80* Pérez AG, Waguespack AM, Binder TD, Southern LL, Falker TM, Ward TL, Steidinger M and Pettigrew JE, 2011. Additivity of effects from dietary copper and zinc on growth performance and fecal microbiota of pigs after weaning. Journal of Animal Science 89, 414– 425. P 85* Shurson GC, Ku PK, Waxler GL, Yokoyama MT and Miller ER, 1990. Physiological relationships between microbiological status and dietary copper levels in the pig. Journal of Animal Science 68, 1061– 1071. P149* Song J, Li YL and Hu CH, 2013. Effect of copper-exchanged montmorillonite, as alternative to antibiotic, on diarrhoea, intestinal permeability and proinflammatory cytokine of weanling pigs. Applied Clay Science 77-78, 52– 55. P103* Varel VH, Robinson IM and Pound WG, 1987. Effect of Dietary Copper Sulfate, Aureo SP250, or Clinoptilolite on Ureolytic Bacteria Found in the Pig Large Intestine. Applied and Environmental Microbiology 53, 2009– 2012. P111* Wang et al., 2012, Effect of copper-loaded chitosan nanoparticles on intestinal microflora and morphology in weaned piglets. Biological Tracer Element Research 149, 184– 189. P116* Xia MS, Hu CH and Xu ZR, 2004. Effect of copper-bearing montmorillonite (Cu-MMT) on Escherichia coli and diarrhoea on weanling pigs. Asian-Austraiasian Journal of Animal Science 17, 1712– 1716. P124* Xia MS, Hu CH and Xu ZR, 2005. Effect of copper bearing montmorillonite on the growth performance, intestinal microflora and morphology of weanling pigs. Animal Feed Science and Technology 118, 507– 517. P123* Yen JT and Nienaber JA, 1993. Effects of high-copper feeding on portal ammonia absorption and oxygen consumption by portal vein-drained organs and whole animal in growing pigs. Journal of Animal Science 71, 2157– 2163. P130* Zhu Y-M, Xie Z-J, Li Y-T and Han X-Y, 2011 Effect of chitosan-copper on growth performance and intestinal flora and its morphology in weanling piglets. Scientia Agricultural Sínica 44, 387– 394. P135* (In Chinese) Alphabetic list of references included in the ELS for chickens (* ID number according to Annex A2) Aydin A, Pekel AY, Issa G, Derimel G and Patterson PH, 2010. Effects of dietary copper, citric acid, and microbial phytase on digesta pH and ileal and crass microbiota of broiler chickens fed a low available phosphorus diet. Journal of Applied Poultry Research 19, 422– 431. CH2* Choi Y and Paik IK, 1989. The effect of supplementing copper sulfate on the performance of broiler chickens. Korean Journal of animal Nutrition and Feedstuffs 13, 193– 200. CH5* (In Korean) Kim CH; Kang HK; Bang HT; Kim JH; Jong H; Choi HC; Paik IK; Moon HK, 2014. Effects of dietary supplementation of copper-sulfate and copper-soy proteinate on the performance and small intestinal microflora in laying hens. Korean Journal of Poultry Science 41, 241– 247. CH15* (In Korean) Kim CH; Shin KS, Kang HK, Kim JH, Jong H, Choi HC; Moon HK; Paik IK; Bang HT, 2014. Effects of supplementary Cu-soy proteinate (Cu-SP) and herbal mixture (HBM) on the growth performance, intestinal microflora, immune response in broilers. Korean Journal of Poultry Science, 41, 165– 171. CH14* (In Korean) Kim BG, Seo YM, Shin KS, Rhee AR, Han J and Paik IK, 2011. Effects of supplemental copper-methionine chelate and copper-soy proteinat on the performance, blood parameters, liver mineral content and intestinal microflora of broiler chickens. Journal of Applied Poultry Research 20, 21– 32. CH16* Kim YK, Chol KH and Paik IK, 1993. Effects of interaction of copper sulfates and dietary fat on the performance of broiler chickens. Korean Journal of Animal Nutrition and Feedstuffs 17, 7– 14. CH18* (In Korean) Ma YL, Xu ZR and Guo T, 2006. Effect of inorganic copper/montmorillonite nanomaterial on growth performance, intestinal microbial flora and bacterial enzyme activities in broilers. Chinese Journal of Animal Science 42, 28– 31. CH 30* (In Chinese) Min SK, Namkung H and Paik IK, 1993. Effects of supplementary copper complexes on the performance of broiler chickens. Korean Journal of Animal Nutrition and Feedstuffs 17, 247– 257. CH34* (In Korean) Min SK, Um JS and Paik IK, 1994. Effect of supplementary methionine-copper and protein-copper complexes on the growth, mineral metabolism and intestinal microflora of broiler chickens and rats. Korean Journal of Animal Nutrition and Feedstuff, 18, 103– 113. CH33* (In Korean) Paik IK, Kim CH and Park KW, 2008. Effect of dietary supplementation of Cu-methionine chelate and Cu-soy proteinate on the performance, small intestinal microflora and immune response in laying hens. Korean Journal of Poultry Science 35, 303– 311. CH40* (In Korean) Pang Y, Patterson JA and Applegate TJ, 2009. The influence of copper concentration and source on ileal microbiota. Poultry Science 88, 586– 592. CH42* Pang Y, Patterson JA and Applegate TJ, 2005. The influence of copper concentration and source on Lactobacilli and E coli populations in batch cultures inoculated with chicken ileal content. Poultry Science 84 Suplement1, 53– 54. CH43* Shi ML, Lan Z and Gue XY, 2013. Copper sciliate nanoparticles: effect of intestinal microflora, nitrogen metabolism and ammonia emission from excreta of yellow-feathered broilers. Chinese Journal of Animal Nutrition 25, 1843– 1850. CH51* (In Chinese) Thomson K, Burkholder K, Patterson J and Applegate TJ, 2008. Microbial ecology shifts in the ileum of broilers during feed withdrawal and dietary manipulations. Poultry Science 87, 1624– 1632. CH56* Xia MS, Hu CH and Xu ZR, 2004. Effects of copper-bearing montmorillonite on growth performance, digestive enzyme activities, and intestinal microflora and morphology of male broilers. Poultry Science 83, 1868– 1875. CH64* Xu ZR, Ma YL and Hu CH, 2003. Effects of CU (II)-exchanged montmorillonite on growth performance, intestinal microflora, bacterial enzyme activities and morphology of broilers. Asian-ustraiasian Journal of Animal Science 16, 1673– 1679. CH65* Zhang CS, Jiang YX, Wang B, Wang RD and Shen HX, 2009. Influence of various dietary copper and vitamin A levels on intestinal structure, cecal gut flora and GH in serum in broilers. Scientia Agricuitura Sinica 42, 1485– 1493. CH67* (in Chinese) Alphabetic list of references included in the ELS for cows (* ID number according to Annex A3) Amachawadi RG, Scott HM, Alvarado CA, Mainini TR, Vinasco J, Droulliard JS and Nagraja TG, 2013. Occurrence of the transferable copper resistance gene tcrB among fecl enterococci of U.S feedlot cattle fed copper-supplemented diets. Applied and Environmental Microbiology 79, 2013 43694375. Cow2* Burke JM, Miller JE, Larsen M and Terrill TH, 2005. Interaction between copper oxide wire particles and Duddingtonia fiagrans in lamb. Veterinary Parasiteiogy 134, 141– 146. Cow13* Jacob ME, Fox JT, Nagaraja, TG, Drouillard, JS, Amachawadi RG and Narayanan SK, 2010. Effects of feeding elevated concentrations of copper and zinc on the antibacterial susceptibilities of fecal bacteria in feedlot cattle. Foodborne Pathogens and Disease 7, 643– 646. Cow31* Janeczek K, Pecka E and Chojnacka K, 2013. Using of biosorption process in obtaining of mineral mixture for sheep and their effects on the selected parameters of rumen fermentation in in vitro conditions. Acta Scientiarum Poionorum - Medicina Veterinaria 12, 5– 13. Cow33* Lilley CW, Hamar DW and Gerlach DW, 1985. Linking copper and bacteria with abomasal ulcers in beef-calves. Veterinary Medicine 80, 85– 88. Cow47* Appendix B - Lists of included/excluded references Alphabetic list of references included in the ELS for pigs and piglets (* ID number according to Annex A1) Armstrong TA, Williams CM, Spears JW and Schiffman SS, 2000. High dietary copper improves odor characteristics or swine waste. Journal of Animal Science 78, 859– 864. P140* Armstrong TA, Cook DR, Ward MM, Williams CM and Spears JE, 2004. Effect of dietary source (cupric citrate and cupric sulphate) and concentration on growth performance and fecal copper excretion in weanling pigs. Journal of Animal Science 82, 1234– 1240. P141* Aviotti MP, deLima FR, Langlois BE, Stahly TS and Cromwell GL, 1980. Effect of single additions and combinations of copper and antibiotics on the performance and fecal coliform resistance patterns of swine. Journal of Animal Science 51 Suppl. 1, 184– 185. P5* Bunch RJ, Speer VC, Hays VW, Hawbaker, JH and Carton DV, 1961. Effects of copper sulfate copper oxide and chlortetracycline on baby pig performance. Journal of Animal Science 20, 723– 726. P142* Dierick NA, Vervaeke IJ, Decuypere JA and Henderickx HK, 1986. Influence of the gut flora and some growth-promoting feed additives on nitrogen-metabolism in pigs. 1. Studies in vitro. Livestock Production Science 14, 161– 176. P 19* Fuller R, Newland LGM, Briggs CAE, Braude R and Mitchell KG, 1960. The normal intestinal flora of the pig. IV. The effect of dietary supplements of penicillin, chlortetracycline or copper sulphate on the fecal flora. Journal of Applied Bacteriology 23, 195– 205. P143* Hawbaker JA, Speer VC, Hays VW and Carton DV, 1961. Effect of copper sulfate and other chemotherapeutics in growing swine rations. Journal of Animal Science 20, 163– 167. P144* Henderickx HK, Vervaeke IJ, Decuypere JA and Dierick NA, 1982. Effect of growth promoting agents on the intestinal gut flora. Advanced Veterinary Medicine 33, 56– 62. P145* Hu CH, Xia MS, Xu ZR and Xiong L, 2004. Effect of copper-bearing montmorillonite on growth performance and digestive function of growing pigs. Asian-Austraiasian Journal of Animal Science 17, 1575– 1581. P46* Huang Y, Zhou TX, Lee JH, Jang HD, Park JC and Kim IH, 2010. Effect of dietary copper sources (Cupric sulfate and cupric methionate) and concentrations on performance and fecal characteristics in growing pigs. Asian-Austraiasian Journal of Animal Science 23, 757– 761. P146* Hojberg O, Canibe N, Poulsen HD and Jensen BB, 2005. Influence of dietary zinc oxide and copper sulfate on the gastrointestinal ecosystem in newly weaned piglets. Applied and Environmental Microbiology 71, 2267– 2277. P41* Jensen BB, 1998. The impact of feed additives on the microbiology of the gut in young pigs. Journal of Animal Feed Science 7, 45– 64. P49* Kellogg TF, Hays VW, Catron DV, Quinn LY and Speer VC, 1966. Effect of dietary chemotherapeutics on the performance and fecal flora of baby pigs. Journal of Animal Science 25, 1102– 1106. P147* Kroger H, Feder H and Plischke R, 1977. Untersuchungen uber den Einfluss verschiedener Cu-Zusatze (Kupfersulfat, Kupfer-II-Oxid und elementares Kupfer) auf die Mast- und Schlachteistungen beim Schwein. Zuchiungskunde 49, 213– 214. P59* (In German) Li R, Lu Y, Hu E-y, Zi Z-h, Sun, G-r and Xia, 2014. Effect of copper glycinate on the fecal copper content and bacterial flora of weanling pig. Acta Agricuiturae Shanghai 30, 15– 18. P148* (In Chinese) Ma YL, Guo T and Xu ZR, 2007. Effect of Cu (II)-exchange montmorillonite on diarrhea incidence, intestinal microflora and mucosa morphology of weaning pigs. Chinese Journal of Veterinary Science 27, 279– 283. P69* (In Chinese) Mei SF, Yu B, Ju CF, Zhu D and Chen DW, 2009. Effect of high levels of dietary zinc and copper on growth performance, digestive physiology and cecal microbe in weanling piglets. Chinese Journal of Animat Nutrition 21, 903– 909. P74* (In Chinese) Mei SF, Yu B, Ju CF, Zhu D and Chen DW, 2010. Effect of different levels of copper on growth performance and cecal ecosystem of newly weaned piglets. Italian Journal of Animal Science 9, 378– 381. P72* Namkung H, Gong H, Yu H and deLange CFM, 2006. Effect of pharmacological intakes of zinc and copper on growth performance, circulating cytokines and gut microbiota of newly weaned piglets, challenged with coliform lipopolysaccharides. Canadian Journal of Animal Science 86, 511– 522. P 80* Pérez AG, Waguespack AM, Binder TD, Southern LL, Falker TM, Ward TL, Steidinger M and Pettigrew JE, 2011. Additivity of effects from dietary copper and zinc on growth performance and fecal microbiota of pigs after weaning. Journal of Animal Science 89, 414– 425. P 85* Shurson GC, Ku PK, Waxler GL, Yokoyama MT and Miller ER, 1990. Physiological relationships between microbiological status and dietary copper levels in the pig. Journal of Animal Science 68, 1061– 1071. P149* Song J, Li YL and Hu CH, 2013. Effect of copper-exchanged montmorillonite, as alternative to antibiotic, on diarrhoea, intestinal permeability and proinflammatory cytokine of weanling pigs. Applied Clay Science 77-78, 52– 55. P103* Varel VH, Robinson IM and Pound WG, 1987. Effect of Dietary Copper Sulfate, Aureo SP250, or Clinoptilolite on Ureolytic Bacteria Found in the Pig Large Intestine. Applied and Environmental Microbiology 53, 2009– 2012. P111* Wang et al., 2012, Effect of copper-loaded chitosan nanoparticles on intestinal microflora and morphology in weaned piglets. Biological Tracer Element Research 149, 184– 189. P116* Xia MS, Hu CH and Xu ZR, 2004. Effect of copper-bearing montmorillonite (Cu-MMT) on Escherichia coli and diarrhoea on weanling pigs. Asian-Austraiasian Journal of Animal Science 17, 1712– 1716. P124* Xia MS, Hu CH and Xu ZR, 2005. Effect of copper bearing montmorillonite on the growth performance, intestinal microflora and morphology of weanling pigs. Animal Feed Science and Technology 118, 507– 517. P123* Yen JT and Nienaber JA, 1993. Effects of high-copper feeding on portal ammonia absorption and oxygen consumption by portal vein-drained organs and whole animal in growing pigs. Journal of Animal Science 71, 2157– 2163. P130* Zhu Y-M, Xie Z-J, Li Y-T and Han X-Y, 2011 Effect of chitosan-copper on growth performance and intestinal flora and its morphology in weanling piglets. Scientia Agricultural Sínica 44, 387– 394. P 135* (In Chinese) Alphabetic list of references excluded in the ELS for pigs and piglets (* ID according to Annex A1) Anonymous, 2015., Northern Ireland disease surveillance report, April to June 2015. Veterinary Record 177, 144– 147. P136* Anonymous, 2012. Quarterly review of diagnostic cases: October to December 2011. Surveillance (Wellington) 39, 14– 22. P137* Anonymous, 2012. Quarterly review of diagnostic cases: April to June 2012. Surveillance (Wellington) 39, 45– 54. P138* Agga G E, Scott H M, Vinasco J, Nagaraja T G, Amachawadi R G, Bai J, Norby B,; Renter, D.G.; Dritz, S.S.; Nelssen, J.L.; Tokach, M.D., 2015. Effects of chlortetracycline and copper supplementation on the prevalence, distribution, and quantity of antimicrobial resistance genes in the fecal metagenome of weaned pigs. Preventive Veterinary Medicine, 119, 179– 189. P1* Almeida AS, Liu Y, Song M, Lee JJ, Gaskins HR, Maddox CW, Osuna O and Pettigrew, J.E., 2013. Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs. Journal of Animal Science and Biotechnology 4, 52. P2* Apgar GA, Kornegay ET, Lindemann MD and Wood CM, 1993. The effect of feeding various levels of Bifidobacterium globosum A on the performance of gastrointestinal measurements, and immunity of weanling pigs and on the performnce and carcass measurements of growing-finishing pigs. Journal of Animat Science 71, 2173– 2179. P3* Aschenbach JR, Ahrens F, Schwelberger HG, Fuerll B, Roesler U, Hensel A and Gaebel G, 2007. Functional characteristics of the porcine colonic epithelium following transportation stress and Salmonella infection. Scandinavian Journal of Gastroenterology 42, 708– 716. P4* Aviotti M P, deRibeiro Lima F, Langlois B E, Stahly T S and Cromwell G L, 1980. Effect of single additions and combinations of copper and antibiotics on the performance and fecal coliform resistance patterns of swine. Journal of Animal Science 51, 184– 185. P5* Awati A, Konstantinov SR, Williams BA, Akkermans ADL, Bosch MW, Smidt H and Verstegen MWA, 2005. Effect of substrate adaptation on the microbial fermentation and microbial composition of faecal microbiota of weaning piglets studied in vitro. Journal of the Science of Food and Agriculture 85, 1765– 1772. P6* Aydin A, Pekel AY, Issa G, Demirel G and Patterson PH, 2010. Effects of dietary copper, citric acid, and microbial phytase on digesta pH and ileal and carcass microbiota of broiler chickens fed a low available phosphorus diet. Journal of Applied Poultry Research 19, 422– 431. P7* Bata A and Kutasi J, Patent. New microelement organic chelate complex compound useful e.g. for inhibiting pathogenic bacteria e.g. Salmonella, and treating or preventing disease e.g. poultry enteric diseases, swine enteric diseases and bovine enteric diseases. WO2014001924-A1; HU201200394-A1; CA2877998-A1; AU2013282911-A1; AR91616-A1; KR2015036295-A; EP2866799-A1; SG11201408685-A1; US2015157594-A1; CN104582697-A; JP2015524388-W. P8* Bolduan G, Jung H, Schneider R and Schnabel E, 1988. Hints on pig nutritionaccording to recent findings of microbial digestion. Monatshefte Fur Veterinarmedizin 43, 764– 766. P9* Bosi P, 1999. Additives and additives. All is not lost. Rivista diSuinicoitura 40, 40– 54. P10* Bourdonnais A, 2015. Mintrex copper: a new chelated copper that delivers more in weaned piglets. Feed Compounder 35, 30– 32. P11* Rowert Research Institute, 1976. Annual report of studies in animal nutrition and allied science 32, 125. P12* Caine WR, Metzler-Zebeli BU, McFall M, Miller B, Ward TL, Kirkwood RN and Mosenthin R, 2009. Supplementation of diets for gestating sows with zinc amino acid complex and gastric intubation of suckling pigs with zinc-methionine on mineral status, intestinal morphology and bacterial translocation in lipopolysaccharide-challenged early-weaned pigs. Research in Veterinary Science 86, 453– 462. P13* Canibe N, Steien SH, Overland M and Jensen BB, 2001. Effect of K-diformate in starter diets on acidity, microbiota, and the amount of organic acids in the digestive tract of piglets, and on gastric alterations. Journal of Animal Science 79, 2123– 2133. P14* Caraivan I and Corina F, 1990. New aspects of prevention and control of dysentery caused by Treponema Serpulina hyodysenteriae and proliferative enteropathy in pigs. Revista Romana de Medicina Veterinara 1, 61– 64. P15* Cassutto B H, Misra HP and Pfeiffer CJ, 1989. Intestinal post-ischemic reperfusion injury - studies with neonatal necrotizing enterocolitis. Acta Physioiogica Hungarica 73, 363– 369. P16* Chen H, Mao X, He J, Yu, B, Huang Z, Yu J, Zheng P and Chen D, 2013. Dietary fibre affects intestinal mucosal barrier function and regulates intestinal bacteria in weaning piglets. British Journal of Nutrition 110, 1837– 1848. P17* Constantinescu C and Paltineanu D, 1976. Study of the effect of iron on the virulence of bacteria. I. Role of ferric ion in experimental Escherichia coli infection. Lucrariie Institutuiui de Cercetari Veterinare si Biopreparate "Pasteur" 11/12, 269– 279. P18* Dominguez VJM, Galvez RN and Marcos MA, Patent. Carmona Rodriguez-Acosta, F.; Rondon RD and Olivares M M. (Patent) New bacterium comprising lactic acid bacterium and bacterium of genus Bifidobacterium comprising metal nanoparticle bound to its surface, useful e.g. as contrast agent for MRI of digestive tract of subject, and for treating cancer. EP2818056-A1; WO2014206969-A1. P20* Dreau D and Lalles JP, 1999. Contribution to the study of gut hypersensitivity reactions to soybean proteins in preruminant calves and early-weaned piglets. Livestock Production Science 60, 209218. P21* Drochner W and Kroger H, 1977. Ddesaturation of organ lipids and tissue-lipids and influence on depot triglyceride structure by feeding high levels of different copper-compounds. Zeitschrift Fur Tierphysioiogie Tierernahrung Und Futtermitteikunde-Journai of Animal Physiology and Animal Nutrition 38, 94– 106. P22* Du J, Wang A, Wang S, Yan G, Yun G and Zhao L, Patent. Composite preblend material for pig capable of improving immunologic function includes ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate, composite microbial ecological agent, ethoxyquinoline, choline chloride and bran. CN102293340-A; CN102293340-B. P23* Edwards AC and Edwards MV, 2111. Nutritional alternatives for antimicrobial control in pigs 2011, 182– 190. P24* Edwards SA, Edge HL, Breuer K, Hillman K, Morgan CA, Stewart A, Strachan WD, Taylor L and Theobald CM, 2007. Agewean - weaning age sorted. Society of Feed Technologists. Proceedings 2007, unpaginated. P25* EFSA Panel on Aditives and Products or Substnces used in Animal Feed (FEEDAP), 2012. Scientific Opinion on the safety and efficacy of copper compounds (E4) as feed additives for all animal species: cupric sulphate pentahydrate based on a dossier submitted by Manica S.p.A. EFSA Journal 10, 2969– 2969. P26* EFSA Panel on Aditives and Products or Substnces used in Animal Feed (FEEDAP), 2012. Scientific Opinion on the safety and efficacy of copper compounds (E4) as feed additives for all animal species: cupric sulphate pentahydrate based on a dossier submitted by Manica S.p.A. EFSA Journal 10, 2969– 2969. P27* Escobar J, Ponder MA, Lessard M, Guay F and Zhao X, 2012 Feeding for gut health in swine. Proceedings of the 33rd Western Nutrition Conference, optimizing efficiency of animal production, Winnipeg, Manitoba, Canada, 19–20 September, 191– 211. P28* Ezerskaya Y A, 2015. How to avoid making mistakes when choosing alternatives to feed antibiotics. Svinovodstvo (Moskva) 2015, 26– 28. P29* Fondevila M, Herrer R, Casallas MC, Abecia L and Ducha