Abstract
1. 1. The circular dichroism (CD) spectra of chromatins of rat liver and AH-130 hepatoma cells were measured, together with those in the presence of the reagents such as sodium dodecyl sulfate, NaCl and urea. Association of DNA with histone in chromatin was supposed to bring about deformation of DNA from the B form to the C form. 2. 2. On increasing the pH of the solution of chromatin, it was supposed that proteins were first disrupted and dissociated from chromatin. As a result, DNA would be subjected to the conformational change from the C form to the B form. The separation of the double helix was then expected. 3. 3. Conformation of DNA in chromatin of AH-130 hepatoma cells was substantially the same as that of normal rat liver.
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