Abstract
From 8 August to 5 December 2006, 1011 individual fish representing twenty species from nineteen different bodies of water throughout New York State were collected and tested by conventional cell culture and viral identification techniques for the presence of Viral Hemorrhagic Septicemia Virus (VHSV). All samples were also tested using a newly developed quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assay. The sampling took place in bodies of water identified by the New York State Department of Environmental Conservation (NYSDEC) as “priority bodies of water” due to the potentially high impact an outbreak of VHSV would have in these waters. Conventional methods returned positive results for two of the twenty fish species: bluntnose minnows ( Pimephales notatus) and emerald shiners ( Notropis artherinoides) from three of the nineteen bodies of water. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) detected VHSV in eight species from eleven bodies of water suggesting greater sensitivity in detecting this pathogen.
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